Gene Structure of Bovine Adrenodoxin Reductase.
نویسندگان
چکیده
منابع مشابه
Chaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli.
Adrenodoxin reductase is an essential component of the mitochondrial monooxygenase systems that are involved in the synthesis of steroid hormones and related compounds. After removing by mutagenesis a secondary ribosome binding site and an mRNA loop formed between the gene and the vector, large amounts of the enzyme could be produced in Escherichia coli by coexpression with the HSP60-chaperone ...
متن کاملThe amino acid sequence of bovine adrenodoxin.
The preliminary amino acid sequence of bovine adrenodoxin previously reported from our laboratory has been shown to be in error. In the corrected sequence, adrenodoxin contains 114 amino acid residues. The residues Asx-GlxLeu in position ‘78, 79, and 80 and the aspartic acid residue in position 107 should be deleted from the previously reported sequence. The details of the primary structure det...
متن کاملPurification and crystallization of NADPH-adrenodoxin reductase from bovine adrenocortical mitochondria.
Flavoprotein and iron sulfur protein as components of an electron transport system have been shown to form a complex under certain conditions [ 1,2]. Chu and Kimura [3] demonstrated that the complex formation between adrenodoxin reductase and adrenodoxin depends on the ionic strength of the environment. We have succeeded in showing the complex formation of adrenodoxin reductase with immobilized...
متن کاملThe structure of adrenodoxin reductase of mitochondrial P450 systems: electron transfer for steroid biosynthesis.
Adrenodoxin reductase is a monomeric 51 kDa flavoenzyme that is involved in the biosynthesis of all steroid hormones. The structure of the native bovine enzyme was determined at 2.8 A resolution, and the structure of the respective recombinant enzyme at 1.7 A resolution. Adrenodoxin reductase receives a two-electron package from NADPH and converts it to two single electrons that are transferred...
متن کاملcAMP post-transcriptionally diminishes the abundance of adrenodoxin reductase mRNA.
Adrenodoxin reductase (AR; ferridoxin: NADP+ oxidoreductase, EC 1.18.1.2) is a flavoprotein that mediates electron transport from NADPH to all known mitochondrial forms of cytochrome P450. AR mRNA was found in all human adult and fetal tissues examined; however, it was vastly more abundant in tissues that synthesize steroid hormones. The ratio of the 18- form of mRNA lacking 18 alternately spli...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 1993
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.16.1200